1. Instituto Nacional de Pediatría
2. INP
3. Artículos Investigadores

Título :	La citotoxicidad inducida por Casiopeína IIgly a las células HeLa agota los niveles de glutatión reducido y es prevenida por el sulfóxido de dimetilo. Casiopeína IIgly induced cytotoxicity to HeLa cells depletes the levels of reduced glutathione and is prevented by dimethyl sulfoxide.
Creador:	FRANCISCO RADAMES ALEMON MEDINA JOSE LUIS MUÑOZ SANCHEZ LENA RUIZ AZUARA MARIA ISABEL GRACIA MORA
Nivel de acceso:	Acceso Abierto
Palabras clave :	MEDICINA Y CIENCIAS DE LA SALUD Antineoplásicos Antineoplásicos - Toxicidad Antioxidantes - Metabolismo Muerte celular - Efecto de drogas Supervivencia celular Citosol - Efectos de drogas Citosol - Ultrastructura Dimetilsulfóxido - Farmacología Colorantes fluorescentes Glutation - Metabolismo Antineoplastic agents - Antagonists & inhibitors Antineoplastic agents - Toxicity Antioxidants - Metabolism Cell death - Drug effects Cell survival - Drug effects Cytosol - Drug effects Cytosol - Ultrastructure Dimethyl sulfoxide - Pharmacology Fluorescent dyes Glutathione - Metabolism LIgly de la casiopeína Daño oxidativo Dihydrorhodamine 123 (DHR 123) Glutatión reducido (GSH) Casiopeína IIgly Oxidative damage Dihydrorhodamine 123 (DHR 123) Reduced glutathione (GSH)
Descripción :	The newly synthesized copper coordination compound Casiopeína IIgly (Cas IIgly) is a promising alternative drug in the treatment of cancer, since it has shown cytotoxicity and genotoxicity in different tumour models. Given its enhanced effects after ascorbic acid-mediated copper reduction, Cas IIgly's activity is thought to be related to oxidative damage. In the present work, oxidized Cas IIgly failed to induce cytosolic oxidative damage in HeLa cells (only 0.9% of the cell population), and in 2.3% of the treated cells when previously reduced, as evaluated through the oxidation of dihydrorhodamine 123 (DHR 123). However, it showed cytotoxicity, since HeLa cells treated with 10-80 microg/mL Cas IIgly proliferated only at 30% of their normal rate, and at 15% when treated with reduced Cas IIgly. This cytotoxicity is strongly abolished in the presence of the hydroxyl scavenger dimethyl sulfoxide. The decrease, from 3994 to 530 nanograms of reduced glutathione (GSH) per million cells after treatment with 80 microg/mL Casiopeína IIgly, indicates that this drug causes the expenditure of this naturally occurring antioxidant. These results altogether suggest that, albeit Cas IIgly induced cytotoxicity is not related to cytosolic DHR 123 oxidation, it may be related to oxidative damage.
Editorial :	Pergamon Press
Fecha de publicación :	2008
Tipo de publicación:	Artículo
Formato:	Adobe PDF
Identificador del Recurso :	10.1016/j.tiv.2007.11.011
Fuente:	Toxicology in Vitro 22(3):710-715
URI :	http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3141
Aparece en las colecciones:	Artículos Investigadores

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