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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3135| Título : | Una estrategia para detectar anomalías cromosómicas en niños con leucemia linfoblástica aguda. / A Strategy to detect chromosomal abnormalities in children with acute lymphoblastic leukemia. |
| Creador: | SILVIA PATRICIA PEREZ VERA SARA FRIAS VAZQUEZ JOSE MIGUEL BETANCOURT RULE MARISA MUJICA SANCHEZ ROBERTO RIVERA LUNA ROCIO ORTIZ LOPEZ |
| Nivel de acceso: | Acceso Abierto |
| Palabras clave : | MEDICINA Y CIENCIAS DE LA SALUD Aberraciones cromosómicas Técnicas de diagnóstico molecular - Métodos Leucemia - Linfoma linfoblástico de células precursoras - Genética Chromosome aberrations Molecular diagnostic techniques - Methods Precursor cell lymphoblastic leukemia-Lymphoma - Genetics Anomalías cromosómicas Leucemia linfoblástica aguda Contenido de ADN Citometría de flujo La fluorescencia de hibridación in situ Chromosomal abnormalities Acute lymphoblastic leukemia DNA content Flow cytometry Fluorescence in situ hybridization |
| Descripción : | Conventional cytogenetics (CC) can be used to identify chromosomal abnormalities that are predictors of treatment outcome in acute lymphoblastic leukemia (ALL). The detection of abnormalities in ALL is difficult because low mitotic index and poor-quality metaphases are obtained. Flow cytometry (FC) and fluorescence in situ hybridization (FISH) can be used to detect aneuploidy in any phaseofthecellcycle,increasingthenumberofanalyzablecells.The aim of this study was to develop a strategy combining these methods toimprovethefrequencyofchromosomeabnormalitydetection.One hundred children with newly diagnosed ALL were included. CC and DNA content analysis by FC were performed in all patients. The numericalabnormalitiesidentifiedbybothmethodswerecomparedand patientswereclassifiedasconcordantordiscordant.FISHwasusedto support aneuploidy results in discrepant cases using centromeric probesforthechromosomesmostfrequentlyinvolvedinaneuploidy. CC and FC showed high concordance (86%). Fourteen cases were discrepant: nine showed hypodiploidy and low hyperdiploidy by cytogenetics and five showed high hyperdiploidy by FC. FISH confirmed aneuploidy in 12 cases in which it could be performed. High hyperdiploidywasthemostcommonabnormality;the31casesshowing this aneuploidy were identified by FC. The search for abnormalities must begin by measuring DNA content to detect this aneuploidy, whichisusefultoevaluatethepatient’srisk.However,itisimportant toscreenforstructuralabnormalitiesbyCCormoleculartechniques. This strategy may detect chromosomal abnormalities, optimizing resourcesinlaboratorieswherenotallthescreeningmethodsareavailable. |
| Editorial : | Lippincott, Williams & Wilkins |
| Fecha de publicación : | 2004 |
| Tipo de publicación: | Artículo |
| Formato: | Adobe PDF |
| Identificador del Recurso : | 10.1097/00043426-200405000-00007 |
| Fuente: | Journal of Pediatric Hematology Oncology 26(5):294 - 300 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3135 |
| Aparece en las colecciones: | Artículos Investigadores |
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| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| artinvest2004_4.pdf | 79.2 kB | Adobe PDF |  Visualizar/Abrir |
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