Por favor, use este identificador para citar o enlazar este ítem:
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2948| Título : | Validation of housekeeping genes as an internal control for gene expression studies in Giardia lamblia using quantitative real-time PCR |
| Creador: | Marcial Quino Jaime |
| Nivel de acceso: | Open access |
| Palabras clave : | Animales Antiprotozoarios - farmacología Cartilla de ADN Regulación de la Expresión Génica Genes Esenciales Giardia lamblia - Efectos de drogas Giardia lamblia - genética Metronidazol - farmacología Reacción en Cadena en Tiempo Real de la Polimerasa Tiazoles - farmacología Animals Antiprotozoal Agents - pharmacology DNA Primers Gene Expression Regulation Genes, Essential Giardia lamblia - drug effects Giardia lamblia - genetics Metronidazole - pharmacology Real-Time Polymerase Chain Reaction Thiazoles - pharmacology G. lamblia; Expresión del gen; Genes de limpieza; RT-qPCR; Programa RefFinder; Validación G. lamblia; Gene expression; Housekeeping genes; RT-qPCR; RefFinder program; Validation |
| Descripción : | The analysis of transcript levels of specific genes is important for understanding transcriptional regulation and for the characterization of gene function. Real-time quantitative reverse transcriptase PCR (RT-qPCR) has become a powerful tool to quantify gene expression. The objective of this study was to identify reliable housekeeping genes in Giardia lamblia. Twelve genes were selected for this purpose, and their expression was analyzed in the wild type WB strain and in two strains with resistance to nitazoxanide (NTZ) and metronidazole (MTZ), respectively. RefFinder software analysis showed that the expression of the genes is different in the three strains. The integrated data from the four analyses showed that the NADH oxidase (NADH) and aldolase (ALD) genes were the most steadily expressed genes, whereas the glyceraldehyde-3-phosphate dehydrogenase gene was the most unstable. Additionally, the relative expression of seven genes were quantified in the NTZ- and MTZ-resistant strains by RT-qPCR, using the aldolase gene as the internal control, and the results showed a consistent differential pattern of expression in both strains. The housekeeping genes found in this work will facilitate the analysis of mRNA expression levels of other genes of interest in G. lamblia. © 2016 Elsevier B.V.. |
| Colaborador(es) u otros Autores: | Fierro Francisco De La Mora-De La Mora Ignacio Enríquez-Flores Sergio Gómez-Manzo Saúl Vanoye-Carlo America Garcia-Torres Itzhel Sierra-Palacios Edgar Reyes-Vivas Horacio |
| Fecha de publicación : | 2016 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1016/j.gene.2016.01.018 |
| Fuente: | Gene 581(1):21 - 30 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2948 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.