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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2902| Título : | The effect of aspartame on rat brain xenobiotic-metabolizing enzymes |
| Creador: | Vences Mejía A |
| Nivel de acceso: | Open access |
| Palabras clave : | Hidrocarburo de Aril Hidroxilasas - metabolismo - Ratas Aspartame - toxicidad - Ratas Encéfalo - efecto de drogas - Ratas Encéfalo - enzimología - Ratas Microsomas - efecto de drogas - Ratas Microsomas - enzimología - Ratas Ratas Wistar Xenobióticos - metabolismo - Ratas Aryl Hydrocarbon Hydroxylases - metabolism - Rats Aspartame - toxicity - Rats Brain - drug effects - Rats Brain - enzymology - rats Microsomes - drug effects - rats Microsomes - enzymology - rats Rats, Wistar Xenobiotics - metabolism - rats Aspartame metabolismo enzimología Xenobióticos ratas aspartame; brain; cytochrome P450; enzyme induction |
| Descripción : | This study demonstrates that chronic aspartame (ASP) consumption leads to an increase of phase I metabolizing enzymes (cytochrome P450 (CYP)) in rat brain. Wistar rats were treated by gavage with ASP at daily doses of 75 and 125 mg/kg body weight for 30 days. Cerebrum and cerebellum were used to obtain microsomal fractions to analyse activity and protein levels of seven cytochrome P450 enzymes. Increases in activity were consistently found with the 75 mg/kg dose both in cerebrum and cerebellum for all seven enzymes, although not at the same levels: CYP 2E1-associated 4-nitrophenol hydroxylase (4-NPH) activity was increased 1.5-fold in cerebrum and 25-fold in cerebellum; likewise, CYP2B1-associated penthoxyresorufin O-dealkylase (PROD) activity increased 2.9- and 1.7-fold respectively, CYP2B2-associated benzyloxyresorufin O-dealkylase (BROD) 4.5- and 1.1-fold, CYP3A-associated erythromycin N-demethylase (END) 1.4- and 3.3-fold, CYP1A1-associated ethoxyresorufin O-deethylase (EROD) 5.5- and 2.8-fold, and CYP1A2-associated methoxyresorufin O-demethylase (MROD) 3.7- and 1.3-fold. Furthermore, the pattern of induction of CYP immunoreactive proteins by ASP paralleled that of 4-NHP-, PROD-, BROD-, END-, EROD- and MROD-related activities only in the cerebellum. Conversely, no differences in CYP concentration and activity were detected in hepatic microsomes of treated animals with respect to the controls, suggesting a brain-specific response to ASP treatment. |
| Colaborador(es) u otros Autores: | Labra-Ruiz N Hernández-Martínez N Dorado-González V Gómez-Garduño J Pérez-López I Nosti-Palacios R Camacho-Carranza R Espinosa-Aguirre Jj |
| Fecha de publicación : | 2006 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1191/0960327106het646oa |
| Fuente: | Hum Exp Toxicol 25(8):453-459 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2902 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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