Por favor, use este identificador para citar o enlazar este ítem:
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2880| Título : | The hexokinase gene family in the zebrafish: structure, expression, functional and phylogenetic analysis |
| Creador: | González Alvarez, R |
| Nivel de acceso: | Open access |
| Palabras clave : | animales Ayuno Perfilación de la Expresión Génica Regulación Enzimológica de la Expresión Génica - efectos de drogas Genoma - genétics Glucosa - administración & dosificación Glucosa - farmacologia Hexoquinasa - química Hexoquinasa - genética Hexoquinasa - metabolismo Humanos Familia de Multigenes Especificidad de Órganos - efectos de drogas Filogenia ARN Mensajero - genetics ARN Mensajero - metabolismo ratas Pez Cebra - genética animals Fasting Gene Expression Profiling Gene Expression Regulation, Enzymologic - drug effects Genome - genetics Glucose - administration & dosage Glucose - pharmacology Hexokinase - chemistry Hexokinase - genetics Hexokinase - metabolism Humans Multigene Family Organ Specificity - drug effects Phylogeny RNA, Messenger - genetics RNA, Messenger - metabolism Rats Zebrafish - genetics Danio rerio, Ayunado, Alimentados con, Glucokinase, Utilización de la glucosa, Hexoquinasa, Evolución Molecular, Hambre Danio rerio Fasted Fed Glucokinase Glucose utilization Hexokinase Molecular evolution Starvation |
| Descripción : | Hexokinase-catalyzed glucose phosphorylation is the first and crucial step for glucose utilization. Although there are reported studies on glucose metabolism in commercial species, knowledge on it is almost nil in zebrafish (Danio rerio), an important model organism for biological research. We have searched these fish hexokinase genes by BLAST analysis; determined their expression in liver, muscle, brain and heart; measured their response to fasting and glucose administration; and performed homology sequences studies to glimpse their evolutionary history. We have confirmed by RT-qPCR studies that the six DNA sequences annotated as possible hexokinases in the NCBI GenBank are transcribed. The organ distribution of the HXK genes is similar in zebrafish as in mammals, to which they are distantly related. Of these, DrGLK and DrSHXK1 are expressed in the fish liver, DrHXK1 in brain and heart, and DrHXK2 in muscle. The only gene responsive to glucose was liver DrGLK. Its expression is induced approximately 1 h after glucose intraperitoneal injection, but not after saline solution injection. The comparison of the fish sequences and the corresponding mammalian ones imply that in both taxa the main muscle and brain isoforms are fusion products of the ancestral gene, their amino halves having separated before than their carboxy ones, followed by the fusion event, whereas fish and mammalian glucokinase genes remained unduplicated |
| Colaborador(es) u otros Autores: | Ortega-Cuellar D Hernández-Mendoza A Moreno-Arriola E Villaseñor-Mendoza K Gálvez-Mariscal A Pérez-Cruz ME Morales-Salas I Velázquez-Arellano A |
| Fecha de publicación : | 2009 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1016/j.cbpb.2008.11.004 |
| Fuente: | Comp Biochem Physiol B Biochem Mol Biol 152(2):189-195 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2880 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.