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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2876| Título : | The effect of nordihydroguaiaretic acid on iodoacetate-induced toxicity in cultured neurons |
| Creador: | Cárdenas Rodríguez, Noemí |
| Nivel de acceso: | Open access |
| Palabras clave : | Inhibidores Enzimáticos - farmacologia - ratones Gliceraldehído-3-Fosfato Deshidrogenasas - antagonistas & inhibidores - ratones Yodoacetatos -toxicidad - ratones Masoprocol - farmacologia - ratones Neuronas - citologia - ratones Neuronas - efectos de drogas - ratones Neuronas - enzimologia - ratones Enzyme Inhibitors - pharmacology - mice Glyceraldehyde-3-Phosphate - Dehydrogenases - antagonists & inhibitors - mice Iodoacetates - toxicity - mice Masoprocol - pharmacology - mice Neurons - cytology - mice Neurons - drug effects - mice Neurons - enzymology - mice Iodoacetato, Ácido Nordihidroguaiarético, Estrés oxidativo Iodoacetate; Nordihydroguaiaretic Acid; Oxidative Stress |
| Descripción : | Nordihydroguaiaretic acid (NDGA) is present in high concentrations in the desert shrub Creosote bush, Larrea tridentate. This plant has been used in traditional medicine because of its beneficial effects related, at least in part, to its antioxidant properties. Taking into account some evidence about neuroprotective effects elicited by NDGA, we evaluated the effect of this compound on the neurotoxicity induced by iodoacetate (IAA), an inhibitor of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), on cerebellar granule neurons. In addition, as reactive oxygen species play an important role in IAA-induced cytotoxicity, we also studied the enzymatic antioxidant system in IAA-treated cells. We found that IAA caused a dose-dependent decrease in cell viability of cultured neurons with an IC50 of 18.4 μM and induced increased activity of catalase, glutathione peroxidase, and glutathione-S-transferase. Moreover, NDGA attenuated the toxicity induced by 18.4, 25, and 30 μM of IAA without abolishing the inhibitory effect of IAA on GAPDH activity. Furthermore, NDGA could prevent the inhibitory effect of IAA on aconitase activity, a marker of oxidative stress, suggesting that the protective effect of NDGA on IAA neurotoxicity was associated with the prevention of oxidative stress. © 2009 Wiley Periodicals, Inc. |
| Colaborador(es) u otros Autores: | Guzmán-Beltrán S Medina-Campos ON Orozco-Ibarra M Massieu L Pedraza-Chaverri J |
| Fecha de publicación : | 2009 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1002/jbt.20278 |
| Fuente: | Journal of Biochemical and Molecular Toxicology 23(2):137 - 142 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2876 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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