Steroidogenic impairment due to reduced ovarian transcription of cytochrome P450 side-chain-cleavage (P450scc) and steroidogenic acute regulatory protein (StAR) during experimental nephrotic syndrome

Peña Rico, M.A.

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Título :	Steroidogenic impairment due to reduced ovarian transcription of cytochrome P450 side-chain-cleavage (P450scc) and steroidogenic acute regulatory protein (StAR) during experimental nephrotic syndrome
Creador:	Peña Rico, M.A.
Nivel de acceso:	Open access
Palabras clave :	Ovario - Metabolismo Fosfoproteínas - biosintesis Esteroides - biosintesis Cholesterol Side-Chain Cleavage Enzyme - biosynthesis Nephrotic Syndrome - enzymology Ovary - metabolism Phosphoproteins - biosynthesis Steroids - biosynthesis Síndrome nefrótico P450scc Estrella esteroidogénesis Nephrotic syndrome P450scc StAR Steroidogenesis
Descripción :	The nephrotic syndrome is a renal disease characterized by proteinuria, hypoproteinemia, edema and hyperlipidemia. It has been reported that female nephrotic rats are characterized by loss of the oestrus cycle, follicle atresia, low gonadotropin and steroid concentrations; particularly, undetectable estradiol levels. Therefore, to determine the mechanisms involved in the ovarian steroidogenesis impairment, in this present study we evaluated the ovarian expression of the essential steroidogenesis components: cytochrome P450 side cholesterol chain cleavage enzyme (P450scc) and steroidogenic acute regulatory protein (StAR). The experiments were conducted in the rat experimental model of nephrosis induced by puromycin aminonucleoside (PAN) and in control groups. The evaluation of the expression of P450scc and StAR mRNA were performed during the acute phase of nephrosis as well as after the exogenous administration of 1 or 4 doses of human chorionic gonadotrophin (hCG), or a daily dose of FSH or FSH + hCG for 10 days. In addition, serum hormone concentrations, intra-ovarian steroid content, and the reproductive capacity were determined. The results revealed a decreased expression of mRNA of P450scc enzyme and StAR during nephrosis, and eventhough they increased after gonadotropins treatment, they did not conduce to a normal cycling rat period or fertility recovery. This study demonstrates that the mechanism by which ovarian steroid biosynthesis is altered during acute nephrosis involves damage at the P450scc and StAR mRNA synthesis and processing.
Colaborador(es) u otros Autores:	Ortiz-Lopez MG Camacho-Castillo L Cardenas M Pedraza- Chaverri J Menjivar M
Fecha de publicación :	2006
Tipo de publicación:	Artículo
Formato:	PDF
Identificador del Recurso :	10.1016/j.lfs.2006.02.023
Fuente:	Life Science 79(7):702-708
URI :	http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2827
Idioma:	eng
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