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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2801| Título : | Serotonin Concentration, Synthesis, Cell Origin, and Targets in the Rat Caput Epididymis During Sexual Maturation and Variations Associated With Adult Mating Status: Morphological and Biochemical Studies |
| Creador: | Jiménez Trejo, Francisco |
| Nivel de acceso: | Open access |
| Palabras clave : | Epidídimo- Citología - ratas Epidídimo - enzimología - ratas Epidídimo - metabolismo - ratas Fenclonina - farmacología - ratas Técnicas para Inmunoenzimas - ratas Ratas Wistar Receptores de Serotonina - metabolismo - ratas Serotonina - Biosíntesis - ratas Serotonina - metabolismo - ratas Conducta Sexual Animal Maduración sexual - ratas Triptófano Hidroxilasa - antagonistas & inhibidores - ratas Triptófano Hidroxilasa - metabolismo - ratas Epididymis - cytology - rats Epididymis - enzymology - rats Epididymis - metabolism - rats Fenclonine - pharmacology - rats Immunoenzyme Techniques - rats Rats, Wistar Receptors, Serotonin - metabolism - rats Serotonin - biosynthesis - rats Serotonin - metabolism - rats Sexual Behavior, Animal Sexual Maturation - rats Tryptophan Hydroxylase - antagonists & inhibitors - rats Tryptophan Hydroxylase - metabolism - rats 5-hydroxytryptamine tryptophan hydroxylase neuroendocrine cells male reproduction serotonin receptor spermatozoa. |
| Descripción : | The caput epididymis of some mammals contains large quantities of serotonin whose origin, targets, and physiological variations have been poorly studied. We combined morphological and biochemical techniques to begin approaching these aspects of serotonin in the rat caput epididymis. Serotonin immunostaining was detected in mast, epithelial, and neuroendocrine cells. Epithelial cells displayed immunoreactivity to 5HT1A, 5HT2A, and 5HT3 serotonin receptors. Endothelial and mast cells labeled positive for 5HT1B serotonin receptors and spermatozoa displayed 5HT2A and 5HT3 serotonin receptor immunoreactivity. Epithelial, endothelial, and mast cells stained positive for serotonin transporters. Only epithelial cells showed tryptophan hydroxylase immunoreactivity; this enzyme catalyzes the limiting step in the serotonin synthetic pathway. In addition, Western blot analyses of caput homogenates documented the presence of 2 protein bands (<51 kd and <48 kd) that were immunoreactive for tryptophan hydroxylase. Chromatographic analyses documented the presence of tryptophan hydroxylase in the caput, and showed that both its activity and serotonin availability increased with sexual maturation and decreased following p-chlorophenylalanine treatment, an inhibitor of tryptophan hydroxylase activity. Interestingly, serotonin concentration and tryptophan hydroxylase activity tended to be higher in breeding males than in those with no mating experience. We think that these results support the existence of a local serotoninergic system in the rat caput epididymis that might regulate some aspects of male reproductive function |
| Colaborador(es) u otros Autores: | Miguel Tapia-Rodríguez Daniel B. C. Queiroz Patricia Padilla María Christina W. Avellar Patricia Rivas Manzano Gabriel Manjarrez-Gutiérrez Gabriel Gutiérrez-Ospina |
| Fecha de publicación : | 2007 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.2164/jandrol.106.000653 |
| Fuente: | Journal of Andrology 28(1):136-149 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2801 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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