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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2648| Título : | NFE2L2 Gene Variants and Arsenic Susceptibility: A Lymphoblastoid Model |
| Creador: | Morales Marin ME |
| Nivel de acceso: | Open access |
| Palabras clave : | Arsénico - toxicidad Proliferación Celular - Efectos de drogas Proliferación Celular - genética Contaminantes Ambientales - toxicidad Regulación de la Expresión Génica - Efectos de drogas Genotipo Humanos Linfocitos B - citología NAD(P)H Deshidrogenasa (Quinona) - genética NAD(P)H Deshidrogenasa (Quinona) - metabolismo Factor 2 Relacionado con NF-E2 - genética Factor 2 Relacionado con NF-E2 - metabolismo Polimorfismo de Nucleótido Simple Reacción en Cadena en Tiempo Real de la Polimerasa Arsenic - toxicity Cell Proliferation - drug effects Cell Proliferation - genetics Environmental Pollutants - toxicity Gene Expression Regulation - drug effects Genotype Humans Lymphocytes - cytology NAD(P)H Dehydrogenase (Quinone) - genetics NAD(P)H Dehydrogenase (Quinone) - metabolism NF-E2-Related Factor 2 - genetics NF-E2-Related Factor 2 - metabolism Polymorphism, Single Nucleotide Real-Time Polymerase Chain Reaction Arsénico Contaminantes Ambientales Nuclear Factor E2 Related Factor 2 SNPs Arsenic Environmental Pollutants NF-E2-Related Factor 2 Polymorphism, Single Nucleotide |
| Descripción : | Arsenic (As) exposure is a major risk for several types of cancer and metabolic diseases such as diabetes. The transcription factor nuclear factor erythroid 2-related factor (Nrf2) is a key mediator in the cellular defense against As-induced adverse effects. The -653G/A and -617C/A gene variants modulate expression levels of the Nrf2 coding gene (NFE2L2) and are postulated to be associated with several illnesses. In this study the functional effect of these polymorphisms was investigated in the cellular sensitivity to As-mediated effects. Using quantitative real-time polymerase chain reaction (qRT-PCR) the basal levels of NFE2L2 mRNA and the induced levels of NFE2L2 and its target gene NQO1 were measured in lymphoblastoid cells carrying different genotypes for -653G/A and -617C/A polymorphisms following As exposure. The effects of different NFE2L2 gene genotypes on cell proliferation were also explored after chronic metal exposure. A tendency toward reduction in basal levels of NFE2L2 mRNA was noted in the heterozygous (GA/CA) and risk homozygous (AA/AA) genotypes of both polymorphisms in immortalized lymphoblastoid cells. Although the expression of NFE2L2 and NQO1 after acute acute iAs exposure was not markedly influenced by -653G/A and -617C/A genotype, it was found that these single-nucleotide polymorphisms (SNPs) were correlated with a differential sensitivity to chronic exposure to the metalloid. Further studies are needed to completely understand the role of -653G/A and -617C/A SNPs in regulation of the NFE2L2 gene. |
| Colaborador(es) u otros Autores: | Mendez-García A Centeno F Martínez-Hernández A Cordova EJ Molina B Frias S Orozco L. 2015 |
| Fecha de publicación : | 2015 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1080/15287394.2015.1004146 |
| Fuente: | Journal of Toxicology and Environmental Health - Part A: Current Issues 78(10):628 - 634 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2648 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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