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Título :	Mouse model of congenital infection with a non-virulent Toxoplasma gondii strain: Vertical transmission, "sterile" fetal damage, or both?
Creador:	Vargas Villavicencio, José Antonio
Nivel de acceso:	Open access
Palabras clave :	Animales Proteínas de Unión al ADN - genética Modelos Animales de Enfermedad Pérdida del Embrión - parasitología femenino Feto - parasitología Feto - patología Hemorragia Transmisión Vertical de Enfermedad Infecciosa masculino Ratones Ratones Consanguíneos BALB C Ratones Consanguíneos C57BL Ratones Noqueados Necrosis Carga de Parásitos Placenta - parasitología Placenta - patología Embarazo Complicaciones Parasitarias del Embarazo - parasitología Complicaciones Parasitarias del Embarazo - patología Organismos Libres de Patógenos Específicos Bazo - parasitología Trombosis Toxoplasmosis Animal -congénito Toxoplasmosis Animal -patología Toxoplasmosis Animal -transmisión Animals DNA-Binding Proteins - genetics Disease Models, Animal Embryo Loss - parasitology Female Fetus - parasitology Fetus - pathology Hemorrhage Infectious Disease Transmission, Vertical Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout Necrosis Parasite Load Placenta/parasitology* Placenta/pathology Pregnancy Pregnancy Complications, Parasitic/parasitology* Pregnancy Complications, Parasitic/pathology Specific Pathogen-Free Organisms Spleen/parasitology Thrombosis Toxoplasmosis, Animal/congenital* Toxoplasmosis, Animal/pathology Toxoplasmosis, Animal/transmission Toxoplasmosis congénita; Modelo de ratón; Carga parasitaria; Patología Congenital toxoplasmosis; Mouse model; Parasitic load; Pathology
Descripción :	Congenital transmission of Toxoplasma gondii may occur if the mother gets infected for the first time while pregnant. The risk of mother-to-child transmission depends on the gestation trimester at infection, being lowest in the first and highest in the last. Conversely, fetal damage is frequent and more severe at the beginning of pregnancy. The objective of this study was to evaluate congenital transmission and pathological aspects in the placenta and the fetus using a mouse model of congenital infection of the second gestation third. Forty-five female BALB/c mice were infected intravenously with 2.5-10.0 × 106 tachyzoites of the ME49 strain at middle gestation. Samples of maternal spleen and fetal/placental units were taken 72 h later. We determined parasite load and vertical transmission by qPCR, as well as damage macroscopically and by histopathology. With the lowest dose, 18% of the fetuses were infected. Also, 40% of fetuses/litter were altered, while this value was 10% in the control group (P < 0.05). These results are similar to those described in humans in terms of vertical transmission and fetal damage during the second third of gestation. The maternal spleen had 10-1000 times more tachyzoites than the placenta, and the later retained 90-99% of the parasites that could reach the fetus. Nevertheless, we found resorptions, abortions or fetal tissue damage in the presence but also in the absence of parasites. Our data indicate a strong protective effect of maternal organs and the placenta against fetal infection, but extensive damage of the later may led to resorption or abortion without vertical transmission. © 2016 Elsevier Inc.
Colaborador(es) u otros Autores:	Cedillo-Peláez Carlos Rico-Torres Claudia Patricia Besné-Mérida A García-Vázquez F Saldaña Ji Correa D
Fecha de publicación :	2016
Tipo de publicación:	Artículo
Formato:	pdf
Identificador del Recurso :	10.1016/j.exppara.2016.04.002
Fuente:	Experimental Parasitology 166():116 - 123
URI :	http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2623
Idioma:	eng
Aparece en las colecciones:	Artículos

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