Por favor, use este identificador para citar o enlazar este ítem:
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2611| Título : | Molecular identification of yeast of the pulque by pcr-dgge, a traditional mexican beverage |
| Creador: | Marcial Quino Jaime |
| Nivel de acceso: | Open access |
| Palabras clave : | Agave - metabolismo Agave - genética Fermentación - génetica Agave - química Etanol - metabolismo Etanol - química Agave - metabolismo Agave - genetics Agave - chemistry Fermentation - genetics Ethanol - metabolism Ethanol - chemistry Pulque PCR Levadura 26SrRNA Pulque PCR Yeast 26SrRNA |
| Descripción : | Currently it is well known that yeasts play an essential role in the production of different beverages. In this paper, were identified some of the yeasts involved in the fermentation process of the pulque, a Mexican traditional beverage. Samples were collected from different regions of Mexico and yeasts were detected directly from samples without cultivation. Identifying the yeasts was obtained using amplification the D1/D2 domain of the 26S rRNA gene and Denaturing Gradient Gel Electrophoresis (DGGE). The results of DGGE showed different profiles of bands in each of the analyzed samples, indicating the presence of several species of yeast, which was also confirmed by sequencing of the bands corresponding to the domain D1/D2, succeeded in identifying five species of yeasts. The results obtained in thiswork demonstrated that the technique used for identification of yeasts of pulque was efficient. Besides, the optimization of this method could also allow rapid identification of yeasts and help understand the role of these in the fermentation process of this beverage, as well as theisolation of strains of interest for biotechnological purposes such as production of ethanol or metabolites with nutraceutical activity |
| Colaborador(es) u otros Autores: | Garcia-Ocón B. Mendoza-Espinoza J.A. Gómez-Manzo S. Enríquez-Flores S And Sierra-Palacios |
| Fecha de publicación : | 2015 |
| Tipo de publicación: | Artículo |
| Fuente: | International Journal of Research -GRANTHAALAYAH 3(3):1-15 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2611 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.