1. Instituto Nacional de Pediatría
2. INP
3. Artículos

Título :	Low expression of IL-6 and TNF-α correlates with the presence of the nuclear regulators of NF-κB, IκBNS and BCL-3, in the uterus of mice.
Creador:	Sierra Mondragón Edith
Nivel de acceso:	Open access
Palabras clave :	Animales Ciclo Estral - genética Ciclo Estral - inmunología Femenino Regulación de la Expresión Génica Interleucina-6 - genética Interleucina-6 - inmunología Ratones Ratones Consanguíneos ICR FN-kappa B - genética FN-kappa B - inmunología Embarazo Proteínas - genética Proteínas - inmunología Transducción de Señales Factores de Transcripción - genética Factores de Transcripción - inmunología Transcripción Genética Factor de Necrosis Tumoral alfa - genética Factor de Necrosis Tumoral alfa - inmunología Útero - inmunología Útero - metabolismo Animals Estrous Cycle - genetics Estrous Cycle - immunology Female Gene Expression Regulation Interleukin-6 - genetics Interleukin-6 - immunology Mice Mice, Inbred ICR NF-kappa B - genetics NF-kappa B - immunology Pregnancy Proteins - genetics Proteins - immunology Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - immunology Signal Transduction Transcription Factors - genetics Transcription Factors - immunology Transcription, Genetic Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - immunology Uterus - immunology Uterus - metabolism NF κ B IκBNS BCL-3 TNF-α IL-6 Estrous NFκB IκBNS BCL-3 TNF-α IL-6 Estrous
Descripción :	The dynamic regulation of NF-κB activity in the uterus maintains a favorable environment of cytokines necessary to prepare for pregnancy throughout the estrous cycle. Recently, the mechanisms that directly regulate the NF-κB transcriptional activity in different tissues are of growing interest. IκBNS and BCL-3 are negative nuclear regulators of NF-κB activity that regulate IL-6 and TNF-α transcription, respectively. Both cytokines have been described as important factors in the remodeling of uterus for blastocyst implantation. In this work we analyzed in ICR mice the mRNA expression and protein production profile of IL-6, TNF-α, and their correspondent negative transcription regulators IκBNS or BCL-3 using real-time PCR, western blot and immunochemistry. We found that the expression of TNF-α and IL-6 was oscillatory along the estrous cycle, and its low expression coincided with the presence of BCL-3 and IκBNS, and vice versa, when the presence of the regulators was subtle, the expression of TNF-α and IL-6 was exacerbated. When we compared the production of TNF-α and IL-6 in the different estrous stages relating with diestrus we found that at estrus there is an important increase of the cytokines (p<0.05) decreasing at metestrus to reach the basal expression at diestrus. In the immunochemistry analysis we found that at diestrus BCL-3 is distributed all over the tissue with a barely detected TNF-α, but on the contrary, at estrus the expression of BCL-3 is not detected with TNF-α clearly observable along the tissue; the same phenomenon occur in the analysis of IκBNS and IL-6. With that evidence we suggest that the expression of TNF-α and IL-6 might be regulated through NF-κB nuclear regulators BCL-3 and IκBNS in the uterus of mice as has been demonstrated in other systems.
Colaborador(es) u otros Autores:	Gómez-Chávez Fernando Murrieta-Coxca Martín Vázquez-Sánchez Ernesto A Martínez-Torres Isaí Cancino-Díaz Marío E Rojas-Espinosa Oscar Cancino Díaz Juan Carlos Reyes-Sánchez José Luis Rodríguez-Muñóz Rafael Rodríguez-Martínez Sandra
Fecha de publicación :	2015
Tipo de publicación:	Artículo
Identificador del Recurso :	10.1016/j.molimm.2015.09.020
Fuente:	Mol Immunol 68(2PtA):333-340
URI :	http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2583
Idioma:	eng
Aparece en las colecciones:	Artículos

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