Induction of oxidative damage by copper-based antineoplastic drugs (Casiopeínas®)

Radame´s Alemo´ n Medina

Por favor, use este identificador para citar o enlazar este ítem: http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2533

Título :	Induction of oxidative damage by copper-based antineoplastic drugs (Casiopeínas®)
Creador:	Radame´s Alemo´ n Medina
Nivel de acceso:	Open access
Palabras clave :	Antineoplásicos - farmacología Cobre - farmacología Células HeLa - Efectos de drogas Células HeLa -metabolismo humans Linfocitos - Efectos de drogas Compuestos Organometálicos - farmacología Estrés Oxidativo - Efectos de drogas Antineoplastic Agents - pharmacology Copper - pharmacology HeLa Cells - drug effects HeLa Cells - metabolism Humans Lymphocytes - drug effects Lymphocytes - metabolism Organometallic Compounds - pharmacology Oxidative Stress - drug effects Casiopeı´NAS medicamentos a base de cobre Daño oxidativo de ROS peroxidación lipídica Genotoxicidad Casiopeı´nas Copper-based drugs ROS Oxidative damage Lipid peroxidation Genotoxicity
Descripción :	Purpose The aim of the present study is to determine in HeLa cells and in human lymphocytes, by an easy and fast method, the induction of oxidative damage to plasma membrane lipids and nuclear DNA by Casiopeı´nas , which are recently synthesized coordination complexes that have been considered as a promising chemotherapeutic alternative for the treatment of cancer, since they have shown cytotoxicity and genotoxicity in several cancer cell lines and xenotransplanted tumours. The presence of an oxidized copper atom in their structure strongly suggests that their mode of action seems to be related to reactive oxygen species (ROS) generation after copper atom reduction through the Fenton and Haber–Weiss system. Method Lipid peroxidation was evaluated as thiobarbituric acid reactive malondialdehyde, cytotoxicity by the fluorescein diacetate/ethidium bromide stain and genotoxicity as DNA fragmentation by the comet assay. Cells were treated with ten different Casiopeı´nas in a concentration range higher than their IC50 (10– 100 lM), both oxidized and reduced in the presence of ascorbic acid
Colaborador(es) u otros Autores:	Matilde Brenda Valle Jose Luis Muñoz Sanchez María Isabel Gracia-Mora Lena Ruiz-Azuara
Fecha de publicación :	2007
Tipo de publicación:	Artículo
Formato:	pdf
Identificador del Recurso :	10.1007/s00280-006-0364-9
Fuente:	Cancer Chemoth Pharm 60(2):219-228
URI :	http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2533
Idioma:	eng
Aparece en las colecciones:	Artículos

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