Por favor, use este identificador para citar o enlazar este ítem:
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2532| Título : | Induction of immunity in sheep to Fasciola hepatica with mimotopes of cathepsin L selected from a phage display library |
| Creador: | Villa Mancera A |
| Nivel de acceso: | Open access |
| Palabras clave : | Catepsinas - inmunología Cisteína Endopeptidasas - inmunología Fascioliasis - veterinaria Biblioteca de Péptidos Enfermedades de las Ovejas - inmunología Cathepsins - immunology Cysteine Endopeptidases - immunology Fascioliasis - veterinary Peptide Library Sheep Diseases - immunology Fasciola hepatica catepsina L mimotopes vacuna Fasciola hepatica cathepsin L mimotopes vaccine |
| Descripción : | An M13 phage random 12-mers peptide library was used to screen cathepsin L mimotopes of Fasciola hepatica and to evaluate their immunogenicity in sheep. Seven clones showed positive reactivity to a rabbit anti-cathepsin L1/L2 antiserum in ELISA, and their amino acid sequences deduced by DNA sequencing were tentatively mapped on the protein. Twenty sheep were randomly allocated into 4 groups of 5 animals each, for immunization with 1x10(14) phage particles of clones 1, 20, a mixture of 7 clones and PBS, without adjuvant at the beginning, and 4 weeks later. All groups were challenged with 300 metacercariae at week 6 and slaughtered 16 weeks later. The mean worm burdens after challenge were reduced by 47.61% and 33.91% in sheep vaccinated with clones 1 and 20, respectively; no effect was observed in animals inoculated with the clone mixture. Also, a significant reduction in worm size and burden was observed for those sheep immunized with clone 1. Animals receiving clone 20, showed a significant reduction in egg output. Immunization induced a reduction of egg viability ranging from 58.92 to 82.11%. Furthermore, vaccinated animals produced clone-specific antibodies which were boosted after challenge with metacercariae of F. hepatica. |
| Colaborador(es) u otros Autores: | Quiroz-Romero H Correa D Ibarra F Reyes-Pérez M Reyes-Vivas H López-Velázquez G Gazarian K Gazarian T Alonso RA |
| Fecha de publicación : | 2008 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1017/S003118200800471X |
| Fuente: | Parasitology 135(12):1437 - 1445 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2532 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.