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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2523| Título : | In vivo expression of helicobacter pylori virulence genes in patients with gastritis, ulcer, and gastric cancer |
| Creador: | Avilés Jiménez F |
| Nivel de acceso: | Open access |
| Palabras clave : | Adulto Antígenos Bacterianos - genética Antígenos Bacterianos - metabolismo Proteínas Bacterianas - genética Proteínas Bacterianas - metabolismo Úlcera Duodenal - microbiología Mucosa Gástrica - microbiología Gastritis - microbiología Regulación Bacteriana de la Expresión Génica Infecciones por Helicobacter - microbiology Helicobacter pylori - genética Helicobacter pylori - patogenicidad Humanos Neoplasias Gástricas - microbiología Virulencia - genética Adult Antigens, Bacterial - genetics Antigens, Bacterial - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Duodenal Ulcer - microbiology Gastric Mucosa - microbiology Gastritis - microbiology Gene Expression Regulation, Bacterial Helicobacter Infections - microbiology Helicobacter pylori - genetics Helicobacter pylori - pathogenicity Humans Stomach Neoplasms - microbiology Virulence - genetics Helicobacter pylori Bacterias Antígenos Proteínas bacterianas Helicobacter pylori Bacteria Antigens Bacterial proteins |
| Descripción : | The best-studied Helicobacter pylori virulence factor associated with development of peptic ulcer disease or gastric cancer (GC) rather than asymptomatic nonatrophic gastritis (NAG) is the cag pathogenicity island (CagPAI), which encodes a type IV secretion system (T4SS) that injects The CagA oncoprotein into host epithelial cells. (RT-PCR) to measure the in vivo expression of genes on the cagPAI and other virulence genes in patients with NAG, duodenal ulcer (DU), or GC. In vivo expression of H. pylori virulence genes was greater overall in gastric biopsy specimens of patients with GC than in those of patients with NAG or DU. However, since in vitro expression of cagA was not greater in H. pylori strains from patients with GC than in those from patients with NAG or DU, increased expression in GC in vivo is likely to result from environmental conditions in the gastric mucosa, though it May in turn cause more severe pathology. Increased expression of virulence genes in GC may represent a stress response to elevated pH or other environmental conditions in the stomach of patients with GC, which may be less hospitable to H. pylori colonization than the acidic environment in patients with NAG or DU The best-studied Helicobacter pylori virulence factor associated with development of peptic ulcer disease or gastric cancer (GC) rather than asymptomatic nonatrophic gastritis (NAG) is the cag pathogenicity island (cagPAI), which encodes a type IV secretion system (T4SS) that injects the CagA oncoprotein into host epithelial cells. Here we used real-time reverse transcription-PCR (RT-PCR) to measure the in vivo expression of genes on the cagPAI and of other virulence genes in patients with NAG, duodenal ulcer (DU), or GC. In vivo expression of H. pylori virulence genes was greater overall in gastric biopsy specimens of patients with GC than in those of patients with NAG or DU. However, since in vitro expression of cagA was not greater in H. pylori strains from patients with GC than in those from patients with NAG or DU, increased expression in GC in vivo is likely a result of environmental conditions in the gastric mucosa, though it may in turn cause more severe pathology. Increased expression of virulence genes in GC may represent a stress response to elevated pH or other environmental conditions in the stomach of patients with GC, which may be less hospitable to H. pylori colonization than the acidic environment in patients with NAG or DU. © 2012, American Society for Microbiology. |
| Colaborador(es) u otros Autores: | Reyes-Leon A Nieto-Patlán E Hansen LM Burgueño J Ramos IP Camorlinga-Ponce M Bermúdez H Blancas JM Cabrera L Ribas-Aparicio RM Solnick JV Torres-López J |
| Fecha de publicación : | 2012 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1128/IAI.05845-11 |
| Fuente: | Infection and Immunity 80(2):594 - 601 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2523 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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