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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2516| Título : | Impairment of glucose metabolism and energy transfer in the hypertriglyceridemic rat heart |
| Creador: | Carvajal Aguilera, Karla Guadalupe |
| Nivel de acceso: | Open access |
| Palabras clave : | Hipertrigliceridemia Metabolismo Energético Fosforilación Oxidativa Glucólisis Consumo de Oxígeno Hypertriglyceridemia Energy Metabolism Oxidative Phosphorylation Glycolysis Oxygen Consumption metabolismo energético fosforilación oxidativa glicolisis consumo de oxígeno función contráctil hypertriglyceridemia Energy metabolism oxidative phosphorylation glycolysis oxygen consumption contractile function hypertriglyceridemia |
| Descripción : | The metabolic pathways involved in ATP production in hypertriglyceridemic rat hearts were evaluated. Hearts from male Wistar rats with sugar-induced hypertriglyceridemia were perfused in an isolated organ system. Mechanical performance, oxygen uptake and beat rate were evaluated under perfusion with different oxidizable substrates. Age- and weight-matched animals were used as control. The hypertriglyceridemic (HTG) hearts showed a decrease in the mechanical work and slight diminution in the oxygen uptake when perfused with glucose, pyruvate or lactate. No differences were found when perfused with palmitate, octanoate or β-hydroxybutyrate. The glycolytic flux in HTG hearts was 2.4 times lower than in control hearts. Phosphofructokinase-I (PFK-I) was 16% decreased in HTG hearts, whereas pyruvate kinase activity did not change. The increased levels of glucose- 6-phosphate in HTG heart, suggested a flux limitation by the PFK-I. Pyruvate dehydrogenase in its active form (PDHa) diminished as well. The PDHa level in the HTG hearts was restored to control values by dichloroacetate; however, this addition did not significantly improve the mechanical performance. Levels of ATP and phosphocreatine as well as total creatine kinase activity and the MB fraction were significant lower in the HTG hearts perfused with glucose. The data suggested that supply of ATP by glucose oxidation did not suffice to support cardiac work in the HTG hearts; this impairment was exacerbated by the diminution of the creatine kinase system output. (Mol Cell Biochem 249: 157–165, 2003) |
| Colaborador(es) u otros Autores: | Baños Guadalupe Moreno Sánchez Rafael |
| Fecha de publicación : | 2003 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1023/A:1024799025126 |
| Fuente: | Molecular And Cellular Biochemistry 249(1 y 2):157-165 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2516 |
| Idioma: | spa |
| Aparece en las colecciones: | Artículos |
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