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Título : Electron microscopy analysis of the nucleolus of Trypanosoma cruzi
Creador: López Velázquez, Gabriel
Nivel de acceso: Open access
Palabras clave : Nucléolo Celular - ultraestructura
Microscopía Electrónica de Transmisión
Proteínas Nucleares - análisis
Trypanosoma cruzi - ultraestructura
Cell Nucleolus - ultrastructure
Microscopy, Electron, Transmission
Nuclear Proteins - Analysis
Trypanosoma cruzi - Ultrastructure
Cuerpos de Cajal
 Nucléolo
 núcleo
 ARN ribosómico
 Trypanosoma cruzi
Cajal bodies
nucleolus
Ribosomal RNA
Trypanosoma cruzi
Descripción : The nucleolus is the main site for synthesis and processing of ribosomal RNA in eukaryotes. In mammals, plants, and yeast the nucleolus has been extensively characterized by electron microscopy, but in the majority of the unicellular eukaryotes no such studies have been performed. Here we used ultrastructural cytochemical and immunocytochemical techniques as well as three-dimensional reconstruction to analyze the nucleolus of Trypanosoma cruzi, which is an early divergent eukaryote of medical importance. In T. cruzi epimastigotes the nucleolus is a spherical intranuclear ribonucleoprotein organelle localized in a relatively central position within the nucleus. Dense fibrillar and granular components but not fibrillar centers were observed. In addition, nuclear bodies resembling Cajal bodies were observed associated to the nucleolus in the surrounding nucleoplasm. Our results provide additional morphological data to better understand the synthesis and processing of the ribosomal RNA in kinetoplastids. © Microscopy Society of America 2005.
Colaborador(es) u otros Autores: Hernández R
López Villaseñor I
Reyes Vivas H
Segura Valdez Mde L
Jiménez García LF
Fecha de publicación : 2005
Tipo de publicación: Artículo
Formato: pdf
Identificador del Recurso : 10.1017/S1431927605050233
Fuente: Microscopy and Microanalysis 11(4):293 - 299
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2391
Idioma: eng
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