Por favor, use este identificador para citar o enlazar este ítem:
http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2354| Título : | A simplified method for hatching and isolating Toxocara canis larvae to facilitate excretory-secretory antigen collection in vitro |
| Creador: | Ponce Macotela, Martha |
| Nivel de acceso: | Open access |
| Palabras clave : | Animales Antígenos Helmínticos - aislamiento y purificación Enfermedades del perro - Parasitología Perros Mujer Larva - Inmunología Óvulo - fisiología Toxocara canis - Inmunología Toxocara canis - aislamiento y purificación Toxocara canis - fisiología Toxocariasis - Parasitología Animals Antigens, Helminth - isolation & purification Dog Diseases - parasitology Dogs Female Larva - immunology Ovum - physiology Toxocara canis - immunology Toxocara canis - isolation & purification Toxocara canis - physiology Toxocariasis - parasitology Giardia excystation Eclosión Toxocara Toxocara excreción / secreción Toxocariasis Giardia excystation Hatching Toxocara Toxocara excretion/secretion Toxocariasis |
| Descripción : | Human toxocariasis causes several dangerous syndromes that can involve the viscera, vision and central nervous system. Diagnosing toxocariasis requires the identification of antibodies against Toxocara canis or Toxocara cati excretions and secretions (ES). To obtain ES it is necessary to collect a large number of larvae. However, since the earliest work describing the culture of Toxocara larvae, few advances in the method have been made. It has been suggested that carbon dioxide triggers molecular mechanisms that enable nematode hatching. A similar hypothesis has been made regarding Giardia excystation. To test the hypothesis we used the Giardia excystation HBSS method to hatch embryonated T. canis eggs. We found that the HBSS method was more effective than the original De Savigny method. Our results suggest that both parasites require stimulation in an acidic environment, and the abrupt change to a basic milieu in duodenum. This physiological adaptation is successful to exploit the intestinal habitat. © 2010 Elsevier B.V. |
| Colaborador(es) u otros Autores: | Rodríguez Caballero Aarón Peralta Abarca Gustavo E Martínez Gordillo Mario N |
| Fecha de publicación : | 2011 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1016/j.vetpar.2010.10.030 |
| Fuente: | Veterinary Parasitology 175(3–4):382 - 385 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2354 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.