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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2309| Título : | A simple and inexpensive in vitro method for retrieving fertilized Toxocara canis eggs |
| Creador: | Rodríguez Caballero, Aarón |
| Nivel de acceso: | Open access |
| Palabras clave : | Larva- crecimiento & desarrollo - perros - México Óvulo - parasitología - perros - México Recuento de Huevos de Parásitos - métodos - perros - México Parasitología - métodos - perros - México Toxocara canis - crecimiento & desarrollo - perros - México Toxocara canis - aislamiento & purificación - perros - México Toxocariasis - Parasitología - perros - México Útero - cirugía - perros - México Cigoto - crecimiento y desarrollo - perros - México Cigoto - secreción - perros - México Larva - growth & development - dogs - Mexico Ovum - parasitology - dogs - Mexico Parasite Egg Count - methods - dogs - Mexico Parasitology - methods - dogs - Mexico Toxocara canis - growth & development - dogs - Mexico Toxocara canis - isolation & purification - dogs - Mexico Toxocariasis - parasitology - dogs - Mexico Uterus - surgery - dogs - Mexico Zygote - growth & developmen- dogs - Mexico Zygote - secretion - dogs - Mexico Animales Enfermedades de los Perros - parasitología Perros Femenino Intestino Delgado - parasitología Larva - crecimiento & desarrollo Óvulo Recuento de Huevos de Parásitos Parasitología - métodos Toxocara canis - crecimiento & desarrollo Toxocara canis - aislamiento & purificación Toxocara canis - parasitología Animals Dog Diseases - parasitology Dogs Female Intestine, Small - parasitology Larva - growth & development Ovum Parasite Egg Count Parasitology - methods Toxocara canis - growth & development Toxocara canis - isolation & purification Toxocariasis - parasitology Uterus - surgery Zygote - growth & development |
| Descripción : | The erratic migration of nematode larvae is potentially deleterious for humans, especially those produced by Toxocara roundworms. Diagnosis of human toxocariosis: visceral larva migrans depends on antibody detection against excretions-secretions (E/S) from Toxocara larvae by enzyme-linked immunosorbent assay. In the present work, we wish to propose a simple and cheap culture procedure for keeping T. canis female worms alive. We removed living worms from the small intestine of euthanized puppies at the Canine Control Centre "Culhuacan" in the Southern part of Mexico City. To ascertain the utility of the method, we compared the population size of fertilized eggs from both female worms kept in culture (FCM) and those obtained using uterus excision method (UEM). Operationally, a fertilized egg develops a larva after 1 month. Our results show that the culture method was superior at a ratio of 3:1 over UEM. FCM had advantages over UEM, such as (1) low probability of contamination with foreign antigens, (2) greater safety for infective egg manipulation, and (3) gave rise to a higher percentage of fertile eggs. © 2007 Springer-Verlag. |
| Colaborador(es) u otros Autores: | Luna-Ochoa, Rodolfo I. Martha Ponce-Macotela Gustavo E. Peralata-Abarca Mario N. Martínez-Gordillo |
| Fecha de publicación : | 2007 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1007/s00436-007-0539-2 |
| Fuente: | Parasitology Research 101(3):829 - 832 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2309 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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