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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2254| Título : | A reliable method to quantify sildenafil and its metabolite N-demethylsildenafil by HPLC in plasma of children |
| Creador: | Ramírez Mendiola, Blanca |
| Nivel de acceso: | Open access |
| Palabras clave : | Niño Cromatografía Líquida de Alta Presión - métodos Humanos Inhibidores de Fosfodiesterasa 5 - sangre Piperazinas - sangre Purinas - sangre Citrato de Sildenafil Sulfonas - sangre Child Chromatography, High Pressure Liquid - methods Humans Phosphodiesterase 5 Inhibitors - blood Piperazines - blood Purines - blood Sildenafil Citrate Sulfones - blood children liquid chromatography pharmacokinetics plasma levels sildenafil |
| Descripción : | The aim of this study was to develop a simple method for quantifying plasma levels of sildenafil and its metabolite by liquid chromatography with a C18 reverse-phase column and UV detection. For both compounds, linearity was assessed in the range from 10 and 1 000 ng · ml-1 and had correlation coefficients of r=0.995 and r=0.997 for sildenafil and its metabolite, respectively. The inter- and intra-day coefficients of variation was<5.3%. The limits of detection and quantification were 1 and 10 ng · ml-1. Drug levels were determined satisfactorily in two patients. A simple and reliable method was developed for use in children with Pulmonary Arterial Hypertension under treatment with sildenafil. © Georg Thieme Verlag KG Stuttgart - New York. |
| Colaborador(es) u otros Autores: | Flores-Pérez C. García Á lvarez R. Juárez-Olguín H. Flores-Pérez J. Chávez Pacheco J.L. |
| Fecha de publicación : | 2013 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1055/s-0033-1345183 |
| Fuente: | Drug Research 63(9):473 - 476 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2254 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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