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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2233| Título : | Cyclosporin-A enhances non-functional axonal growing after complete spinal cord transection |
| Creador: | Ibarra, Antonio |
| Nivel de acceso: | Open access |
| Palabras clave : | Axones - efectos de drogas - ratas Ciclosporina - farmacología - ratas Proteína GAP-43 - biosíntesis - ratas Proteína GAP-43 - efectos de drogas - ratas Inmunohistoquímica - métodos - ratas Regeneración Nerviosa - efectos de drogas - ratas Fármacos Neuroprotectores - farmacología - ratas Tractos Piramidales - efectos de drogas - ratas Ratas Sprague-Dawley Traumatismos de la Médula Espinal - ratas Axons - drug effects - rats Cyclosporine - pharmacology - rats GAP-43 Protein - biosynthesis - rats GAP-43 Protein - drug effects - rats Immunohistochemistry - methods - rats Nerve Regeneration - drug effects - rats Neuroprotective Agents - pharmacology - rats Pyramidal Tracts - drug effects - rats Rats, Sprague-Dawley Spinal Cord Injuries - rats GAP-43 Motor recovery Neuroregeneration Somatosensory evoked potential Spinal cord injury Therapy |
| Descripción : | Therapeutic approaches that promote both neuroprotection and neuroregeneration would be valuable for spinal cord (SC) injury therapies. Cyclosporin-A (CsA) is an immunosuppressant that, due to its mechanism of action, could both protect and regenerate the neural tissue after injury. Previous studies have already demonstrated that intraperitoneal administration of CsA at a dose of 2.5 mg/kg/12 h during the first 2 days after SC contusion, followed by 5 mg/kg/12 h orally, diminishes tissue damage and improves motor recovery. In order to evaluate the effect of this CsA dosing regimen on axonal growth, we assessed motor recovery, presence of axons establishing functional connections and expression of GAP-43 in rats subjected to a complete SC transection. The Basso-Beattie-Bresnahan rating scale did not show difference in motor recovery of CsA or vehicle-treated rats. Moreover, somato-sensorial evoked potentials demonstrated no functional connections in the SC of these animals. Nevertheless, histological studies showed that: i) a significant number of CsA-treated rats presented growing axons, although they deviated perpendicularly at the edge of the stumps, surrounding them, ii) the expression of GAP-43 in animals treated with CsA was higher than that observed in the control group. Finally, anterograde tracing of the corticospinal tract of rats subjected to an incomplete SC transection showed no axonal fibers reaching the caudal stump. In summary, CsA administered at the dosing-regimen that promotes neuroprotection in SC contused rats induces both GAP-43 expression and axonal growth; however, it failed to generate functional connections in SC transected animals. © 2007 Elsevier B.V. All rights reserved. |
| Colaborador(es) u otros Autores: | Edson Hernández Joel Lomelic Dante Pineda Maribel Buenrostro Susana Martiñón Elisa Garcia Nayeli Floresa Gabriel Guizar Sahaguna Dolores Correa Ignacio Madrazo |
| Fecha de publicación : | 2007 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1016/j.brainres.2007.02.056 |
| Fuente: | Brain Research 1149(1):200 - 209 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2233 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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