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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2143| Título : | A new method to quantify ifosfamide blood levels using dried blood spots and UPLC-MS/MS in paediatric patients with embryonic solid tumours |
| Creador: | Torres Espíndola, Luz María |
| Nivel de acceso: | Open access |
| Palabras clave : | Adolescente Niño Preescolar Cromatografía Líquida de Alta Presión - métodos Ciclofosfamida Demografía Pruebas con Sangre Seca - métodos Femenino Hematócrito Humanos Ifosfamida - sangre Lactante Recién nacido Masculino Neoplasias de Células Germinales y Embrionarias - sangre Reproducibilidad de Resultados Espectrometría de Masas en Tándem - métodos Adolescent Child Child, Preschool Chromatography, High Pressure Liquid - methods Cyclophosphamide Demography Dried Blood Spot Testing - methods Female Hematocrit Humans Ifosfamide - blood Infant Infant, Newborn Male Neoplasms, Germ Cell and Embryonal - blood Reproducibility of Results Tandem Mass Spectrometry - methods Ifosfamida Ciclofosfamida sangre niño cromatografía Tumor de Célula Germinal Espectrometría de Masa en Tándem Ifosfamide Cyclophosphamide blood child Chromatography Neoplasms, Germ Cell and Embryonal Tandem Mass Spectrometry |
| Descripción : | Ifosfamide blood concentrations are necessary to monitor its therapeutic response, avoiding any adverse effect. We developed and validated an analytical method by UPLC-MS/MS to quantify ifosfamide in dried blood spots (DBS). Blood samples were collected on Whatman 903® filter paper cards. Five 3 mm disks were punched out from each dried blood spot. Aceto-nitrile and ethyl acetate were used for drug extraction. Chromatographic separation was carried out in an Acquity UPLC equipment with a BEH-C18 column, 2.1 × 100 mm, 1.7 μm (Waters®). The mobile phase consisted in 5 mM ammonium formate and methanol: acetonitrile (40:48:12 v/v/v) at 0.2 mL/min. LC-MS/MS detection was done by ESI+ and multiple reaction mode monitoring, ionic transitions were m/z1+ 260.99 > 91.63 for ifosfamide and 261.00 > 139.90 for cyclo-phosphamide (internal standard). This method was linear within a 100-10000 ng/mL range and it was accurate, precise and selective. Ifosfamide samples in DBS were stable for up to 52 days at -80°C. The procedure was tested in 14 patients, ages 1 month to 17 years (9 males and 5 females), with embryonic tumours treated with ifosfamide, alone or combined, at a public tertiary referral hospital. Ifosfamide blood levels ranged from 11.1 to 39.7 μmol/L at 12 hours after the last infusion, while 24-hour levels ranged from 0.7-19.7 μmol/L. The median at 12 hours was 19.5 μmol/L (Q25 14.4-Q7529.0) and 3.8 μmol/L (Q25 1.5-Q75 9.9) at 24 hours, p<0.001. This method is feasible to determine ifosfamide plasma levels in paediatric patients. © 2015 Torres et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Colaborador(es) u otros Autores: | Rivera-Espinosa L Chávez-Pacheco JL Navas Cf Demetrio Ja Alemón-Medina R Trujillo F Pérez M Zapata M Cárdenas R Salinas C Arnoldo Ar Velázquez Cr Castillejos Mj. |
| Fecha de publicación : | 2015 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1371/journal.pone.0143421 |
| Fuente: | Plos One 10(11):1-13 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2143 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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