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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2122| Título : | Biotin regulates the genetic expression of holocarboxylase synthetase and mitochondrial carboxylases in rats |
| Creador: | Rodríguez Meléndez, Rocío |
| Nivel de acceso: | Open access |
| Palabras clave : | Animales Biotina - deficiencia Biotina - farmacología Ligasas de Carbono-Nitrógeno - metabolismo Carboxiliasas - efectos de drogas Carboxiliasas - genética Regulación Enzimológica de la Expresión Génica - efectos de drogas ARN Mensajero - efectos de drogas Animals Biotin- deficiency Biotin - pharmacology Carbon-Nitrogen Ligases - metabolism Carboxy-Lyases - drug effects Carboxy-Lyases - genetics Gene Expression Regulation, Enzymologic - drug effects RNA, Messenger - drug effects biotina carboxilasas holocarboxilasa sintetasa expresión génica ratas Biotin carboxylases holocarboxylase synthetase gene expression rats |
| Descripción : | Biotin is the cofactor of carboxylases [pyruvate (PC), propionyl-CoA (PCC), 3-methyl crotonyl-CoA and acetyl-CoA], to which it is covalently bound by the action of holocarboxylase synthetase (HCS). We have studied whether biotin also regulates their expression, as it does other, nonrelated enzymes (e.g., glucokinase, phosphoenol pyruvate carboxykinase, guanylate cyclase). For this purpose, HCS, PC and PCC mRNAs were studied in biotin-deficient rat liver, kidney, muscle and brain of biotin-deficient rats. PC- and PCC-specific activities and protein masses were also measured. The 24-h time course of HCS mRNA in deficient rats was examined after biotin supplementation. HCS mRNA was significantly reduced during vitamin deficiency. It increased in deficient rats after biotin was injected, reaching control levels 24 h after administration. These changes seem to be the first known instance in mammals of an effect of a water-soluble vitamin on a mRNA functionally related to it. In contrast, the decreased activities of the carboxylases were associated with reductions in the amounts of their enzyme proteins except in brain. However, their mRNA levels were not affected. There are no reports on these types of vitamin affecting the mRNA or protein levels of their apoenzymes or their products. This work provides evidence for biotin being a modulator of the genetic expression of the enzymes involved in its function as a cofactor. As such, it may be a useful model for probing a similar role for other water-soluble vitamins. |
| Colaborador(es) u otros Autores: | Cano Saúl Méndez Sara Teresa Velázquez Antonio |
| Fecha de publicación : | 2001 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Fuente: | Journal of Nutrition 131(7):1909 - 1913 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/2122 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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