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Título :	Animal model of acute gout reproduces the inflammatory and ultrasonographic joint changes of human gout
Creador:	Pineda C.,
Nivel de acceso:	Open access
Palabras clave :	Enfermedad Aguda Animales Artritis Gotosa - diagnóstico por imagen Artritis Gotosa - metabolismo Artritis Gotosa - patología Biomarcadores - metabolismo Modelos Animales de Enfermedad Humanos Masculino Osteoartritis de la Rodilla - diagnóstico por imagen Osteoartritis de la Rodilla - metabolismo Osteoartritis de la Rodilla - patología Conejos Líquido Sinovial - citología Líquido Sinovial - metabolismo Ultrasonografía Acute Disease Animals Arthritis, Gouty - diagnostic imaging Arthritis, Gouty - metabolism Arthritis, Gouty - pathology Biomarkers - metabolism Disease Models, Animal Humans Male Osteoarthritis, Knee - diagnostic imaging Osteoarthritis, Knee - metabolism Osteoarthritis, Knee - pathology Rabbits Synovial Fluid - cytology Synovial Fluid - metabolism Ultrasonography Gota Ácido Úrico Líquido Sinovial Inflamación Conejos patología Gout Urato Monosódico Synovial Fluid Inflammation rabbit Pathology
Descripción :	Introduction: Gout is an inflammatory condition induced by the deposition of monosodium urate (MSU) crystals in the joints and soft tissues that can produce acute or chronic arthritis. Several animal models of crystal-induced inflammation have been proposed that involve direct injection of MSU-crystals into different anatomical structures; however, only a few of these models reflect a true diarthrodial joint microenvironment in which an acute gouty attack takes place. The aim of this study was to assess the inflammatory and structural joint changes in a rabbit model of acute gout attack by ultrasound (US), synovial fluid (SF) and histopathological analyses. Methods: Under US guidance, 42 rabbit knees were randomly injected with a suspension of 50 mg/ml of either MSU or allopurinol synthetic crystals. The control group received intra-articular vehicle of phosphate-buffered saline (PBS). US evaluation, SF and histopathological analyses were performed at days 1, 3, and 7. Results: A total of 21 rabbit knees were assigned to the control group, 12 to the MSU-crystals group, and 9 to the allopurinol crystals group. By US, the MSU crystals group displayed the double contour sign and bright stippled aggregates in 67% and 75% of joints, respectively. Neither control knees nor allopurinol crystals group displayed these US signs. Power Doppler (PD) signal was moderate to intense in the MSU-crystals group and greater than both the allopurinol crystal and control groups at day 1 (P <0.001) and 3 (P <0.05), with its practical disappearance by day 7. SF leukocyte count was 40,312 ± 6,369 cells/mm3 in the MSU-crystals group, higher than in controls (P = 0.004) and allopurinol crystal group (P = 0.006). At day 7, SF leukocyte count decreased in both MSU and allopurinol crystal groups reaching the non-inflammatory range. Histologically, at day 3 intense synovial polymorphonuclear cells infiltration and MSU aggregates were identified. Conclusion: The rabbit model of MSU crystal-induced acute arthritis efficiently reproduces the inflammatory, US, SF and histopathological changes of the human acute gouty attack. © 2015 Pineda et al.
Colaborador(es) u otros Autores:	Fuentes-Gómez A.J. Hernández-Díaz C. Zamudio-Cuevas Y. Fernández-Torres J. López-Macay A. Alba-Sánchez I. Camacho-Galindo J. Ventura L. Gómez-Quiróz L.E. Gutiérrez-Ruiz M.C. García-Vázquez F. Reginato A.M. Gutiérrez M. López-Reyes A.
Fecha de publicación :	2015
Tipo de publicación:	Artículo
Formato:	pdf
Identificador del Recurso :	10.1186/s13075-015-0550-4
Fuente:	Arthritis Research and Therapy 17(1):2-9
URI :	http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/1559
Idioma:	eng
Aparece en las colecciones:	Artículos

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