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Título : Animal model of acute gout reproduces the inflammatory and ultrasonographic joint changes of human gout
Creador: Pineda C.,
Nivel de acceso: Open access
Palabras clave : Enfermedad Aguda
Animales
Artritis Gotosa - diagnóstico por imagen
Artritis Gotosa - metabolismo
Artritis Gotosa - patología
Biomarcadores - metabolismo
Modelos Animales de Enfermedad
Humanos
Masculino
Osteoartritis de la Rodilla - diagnóstico por imagen
Osteoartritis de la Rodilla - metabolismo
Osteoartritis de la Rodilla - patología
Conejos
Líquido Sinovial - citología
Líquido Sinovial - metabolismo
Ultrasonografía
Acute Disease
Animals
Arthritis, Gouty - diagnostic imaging
Arthritis, Gouty - metabolism
Arthritis, Gouty - pathology
Biomarkers - metabolism
Disease Models, Animal
Humans
Male
Osteoarthritis, Knee - diagnostic imaging
Osteoarthritis, Knee - metabolism
Osteoarthritis, Knee - pathology
Rabbits
Synovial Fluid - cytology
Synovial Fluid - metabolism
Ultrasonography
Gota
Ácido Úrico
Líquido Sinovial
Inflamación
Conejos
patología
Gout
Urato Monosódico
Synovial Fluid
Inflammation
rabbit
Pathology
Descripción : Introduction: Gout is an inflammatory condition induced by the deposition of monosodium urate (MSU) crystals in the joints and soft tissues that can produce acute or chronic arthritis. Several animal models of crystal-induced inflammation have been proposed that involve direct injection of MSU-crystals into different anatomical structures; however, only a few of these models reflect a true diarthrodial joint microenvironment in which an acute gouty attack takes place. The aim of this study was to assess the inflammatory and structural joint changes in a rabbit model of acute gout attack by ultrasound (US), synovial fluid (SF) and histopathological analyses. Methods: Under US guidance, 42 rabbit knees were randomly injected with a suspension of 50 mg/ml of either MSU or allopurinol synthetic crystals. The control group received intra-articular vehicle of phosphate-buffered saline (PBS). US evaluation, SF and histopathological analyses were performed at days 1, 3, and 7. Results: A total of 21 rabbit knees were assigned to the control group, 12 to the MSU-crystals group, and 9 to the allopurinol crystals group. By US, the MSU crystals group displayed the double contour sign and bright stippled aggregates in 67% and 75% of joints, respectively. Neither control knees nor allopurinol crystals group displayed these US signs. Power Doppler (PD) signal was moderate to intense in the MSU-crystals group and greater than both the allopurinol crystal and control groups at day 1 (P <0.001) and 3 (P <0.05), with its practical disappearance by day 7. SF leukocyte count was 40,312 ± 6,369 cells/mm3 in the MSU-crystals group, higher than in controls (P = 0.004) and allopurinol crystal group (P = 0.006). At day 7, SF leukocyte count decreased in both MSU and allopurinol crystal groups reaching the non-inflammatory range. Histologically, at day 3 intense synovial polymorphonuclear cells infiltration and MSU aggregates were identified. Conclusion: The rabbit model of MSU crystal-induced acute arthritis efficiently reproduces the inflammatory, US, SF and histopathological changes of the human acute gouty attack. © 2015 Pineda et al.
Colaborador(es) u otros Autores: Fuentes-Gómez A.J.
Hernández-Díaz C.
Zamudio-Cuevas Y.
Fernández-Torres J.
López-Macay A.
Alba-Sánchez I.
Camacho-Galindo J.
Ventura L.
Gómez-Quiróz L.E.
Gutiérrez-Ruiz M.C.
García-Vázquez F.
Reginato A.M.
Gutiérrez M.
López-Reyes A.
Fecha de publicación : 2015
Tipo de publicación: Artículo
Formato: pdf
Identificador del Recurso : 10.1186/s13075-015-0550-4
Fuente: Arthritis Research and Therapy 17(1):2-9
URI : http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/1559
Idioma: eng
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