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http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/1282| Título : | AMP-activated protein kinase regulates oxidative metabolism in Caenorhabditis elegans through the NHR-49 and MDT-15 transcriptional regulators |
| Creador: | Moreno Arriola, Elizabeth |
| Nivel de acceso: | Open access |
| Palabras clave : | Aminoimidazol Carboxamida - análogos & derivados Aminoimidazol Carboxamida -farmacología Animales Caenorhabditis elegans - Efectos de drogas Caenorhabditis elegans - genética Caenorhabditis elegans - metabolismo Proteínas de Caenorhabditis elegans - genética Proteínas de Caenorhabditis -metabolismo Respiración de la Célula - Efectos de la droga Metabolismo Energético - Efectos de drogas Metabolismo Energético - genética Ácidos Grasos - metabolismo Regulación de la Expresión Génica Glucosa - metabolismo Humanos Ácido Láctico - metabolismo Longevidad - genética Metformina - farmacología Mitocondrias Efectos de drogas Mitocondrias -metabolismo Oxígeno - metabolismo Consumo de Oxígeno - efectos de drogas Subunidades de Proteína - genética Subunidades de Proteína - metabolismo Proteínas Serina-Treonina Quinasas - genética Proteínas Serina-Treonina Quinasas - metabolismo Receptores Citoplasmáticos y Nucleares - genética Receptores Citoplasmáticos y Nucleares - metabolismo Ribonucleótidos -farmacología Transducción de Señal Factores de Transcripción - genética Factores de Transcripción - metabolismo Transcripción Genética Aminoimidazole Carboxamide - analogs & derivatives Aminoimidazole Carboxamide - pharmacology Animals Caenorhabditis elegans - drug effects Caenorhabditis elegans - genetics Caenorhabditis elegans - metabolism Caenorhabditis elegans Proteins - genetics Caenorhabditis elegans Proteins - metabolism Cell Respiration - drug effects Energy Metabolism - drug effects Energy Metabolism - genetics Fatty Acids - metabolism Gene Expression Regulation Glucose - metabolism Humans Lactic Acid - metabolism Longevity - genetics Metformin - pharmacology Mitochondria - drug effects Mitochondria - metabolism Oxygen - metabolism Oxygen Consumption - drug effects Protein Subunits - genetics Protein Subunits - metabolism Protein-Serine-Threonine Kinases - genetics Protein-Serine-Threonine Kinases - metabolism Receptors, Cytoplasmic and Nuclear - genetics Receptors, Cytoplasmic and Nuclear - metabolism Ribonucleotides - pharmacology Signal Transduction Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic Caenorhabditis elegans, Proteínas de C elegans, Subunidades de Proteína, Proteínas Serina-Treonina Quinasas, Receptores Citoplasmáticos y Nucleares, Factores de Transcripción, genética Caenorhabditis elegans, Caenorhabditis elegans Proteins, Protein Subunits, Protein-Serine-Threonine Kinases, Receptors, Cytoplasmic and Nuclear, Transcription Factors, genetics |
| Descripción : | Cellular energy regulation relies on complex signaling pathways that respond to fuel availability and metabolic demands. Dysregulation of these networks is implicated in the development of human metabolic diseases such as obesity and metabolic syndrome. In Caenorhabditis elegans the AMP-activated protein kinase, AAK, has been associated with longevity and stress resistance; nevertheless its precise role in energy metabolism remains elusive. In the present study, we find an evolutionary conserved role of AAK in oxidative metabolism. Similar to mammals, AAK is activated by AICAR and metformin and leads to increased glycolytic and oxidative metabolic fluxes evidenced by an increase in lactate levels and mitochondrial oxygen consumption and a decrease in total fatty acids and lipid storage, whereas augmented glucose availability has the opposite effects. We found that these changes were largely dependent on the catalytic subunit AAK-2, since the aak-2 null strain lost the observed metabolic actions. Further results demonstrate that the effects due to AAK activation are associated to SBP-1 and NHR-49 transcriptional factors and MDT-15 transcriptional co-activator, suggesting a regulatory pathway that controls oxidative metabolism. Our findings establish C. Elegans as a tractable model system to dissect the relationship between distinct molecules that play a critical role in the regulation of energy metabolism in human metabolic diseases. © 2016 Moreno-Arriola et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Colaborador(es) u otros Autores: | Mohammed ELHafidi Daniel Ortega-Cuéllar Karla Carvajal |
| Fecha de publicación : | 2016 |
| Tipo de publicación: | Artículo |
| Formato: | |
| Identificador del Recurso : | 10.1371/journal.pone.0148089 |
| Fuente: | Plos One 11(1):1 - 20 |
| URI : | http://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/1282 |
| Idioma: | eng |
| Aparece en las colecciones: | Artículos |
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